This assay method was also applied in vivo in the rat. Commitment functions and their dependence upon pH calculated in this connection appear to be in agreement with known kinetic parameters of liver alcohol dehydrogenase. It amounts to about 10 with NAD+ and about 5 with the coenzyme analogues. The intrinsic isotope effect evaluated by the method of Northrop varies little with pH. With increasing pH, these values decreased to 1.5 and 2.5 at pH 9.

With NAD+ at pH 7, D(V/K) was 3.0 and T(V/K) was 6.5. V/K isotope effects for both tritium and deuterium have been measured with three different coenzymes, NAD+, thio-NAD+, and acetyl-NAD+. Synthesis of the necessary labeled compounds is described in this and earlier work referred to. 14C and 3H, to follow the rate of acetaldehyde formation from deuterium-labeled ethanol and normal ethanol, respectively. The deuterium isotope effect was obtained with high reproducibility by the use of two different radioactive tracers, viz. The primary isotope effect upon V/K when ethanol stereospecifically labeled with deuterium or tritium is oxidized by liver alcohol dehydrogenase has been measured between pH 6 and 9. Julie Thomson Katherine Popham Kathleen Hinrichs Kathleen Rood Katie Nickels Kimberly Bruder Larry Folden Leonard (Leo) Sausen Linda Hawkinson Lois Dupre Margaret (Peggy) Durkee Mark Schmidt Marlys Tews Mary.Primary deuterium and tritium isotope effects upon V/K in the liver alcohol dehydrogenase reaction with ethanol. Richard Swanberg Rich Lehmann Rita Arth Shannon Kiesling Sheila Carrell Sherilyn Huerstel Sheryl Anderson Steven (Steve) Hagen Steven (Steve) Knutsen Steven (Steve) Linnerooth Susan Herzog Susan Moran Terry Moen Veronica Carlson Vicki Tomlinson Victor Palmateer William 'Bill' Lawler William Dalin William KunshierĪrlene Puro Barbara Nelson Bradley (Brad) Simons Brenda Dotray Brenda Lundin Bruce Johnson Bruce Weinke Candy Bohjanen Candy Larson Carol Pfingsten Catherine (Cathy) Scofield Cheryle Gudmundsen Clifford Wickman Cynthia Cindy Lawrence Cynthia(cindy) Lawrence Dale Perreault David Witham Deborah Larson Debra Branum Debra Houleĭee Lindstrom Diane Anderson Donald Omundson Douglas (Doug) Hallberg Duane Maki Ericson Linscot Garman Torp Gary Lundeen Gary Simonson George Mickelsen Gerald Monson Gordy Grubbs Grace Peterson James 'Jim' Swanberg Jane Jones Jan Peters Jeffrey Renard Joellen Hansen Joel Skelton John Long

Judy Quammen Julane Nielsen Kathleen Eckdahl Laurie Magnuson Linda Mueller Margaret Miller Marjorie Danchenko Mark Pogreba Marla Rosenberg Mary Campbell Michele Berggren Pamela (Pam) Peterson Pamela Taurman Patricia Johnson Patrick McMurchie Paudeen Goodell Randy Heath Rebecca (Becky) Brown Rebecca Paine Richard Carlson Patti Hayes Peter Flesvig Phillip Madison Richard Rasmussen Ronald James Larson Ronald Olsen Ruth Goss Sandy Johnson Stephen McKinnon Susan MacKey Susan Osterbauer Theodore (Ted) Miller Thomas Sundboom Timothy McconnellĪllen Talvitie Angela Dolphy Arlen Swanson Barbara Cockburn Barbara Johnson Barbara Kreuscher Barbra Heglson Candace Diemert Candace Nadeau Carol Jorgenson Catherine (Cathy) Knudsen Cathrine Knudsen Charlotte Ratzlaff Cheryl Solem Chris Ross Christine Haluptzok Clude Wason Connie Videen Cyril Riopel Daniel Christensenĭaniel Sandberg Dan Poff Dave Peterson Deborah Bond Debra Johnson Delores Dagostino Earl Fashbaugh Frank Pruden Gregory Johnson Iris Vogel James Cartier Jerry Thompson John Bunn John Rice Jonathon (Jon) Pfingsten Joseph (Joe) Waldoch Joyce Jeans Judie Johnston Judith Dupre Judy Freshwater Lynn Carlson Margaret (Peggy) Doyle Mark Hanson Mark Lellman Marti Seeley Mary Ann Donovan Mary Berven Melvin Stoltzman Michael (Mike) Heath Michael San Cartier Muriel Wickman Nancy Blad Pamela Frandle Patricia Houle Patricia Sederholm Patricia Strandberg Alan Davis Ann Miller Barbara Marier Barbara Peterson Carole Berry Catherine Augustine Charles 'Charlie' McConnell Christine Hult Cindy Pepin Dulac Court Nelson Craig Lindstrom Cynthia Pepin Cynthia Sahlin Dana Berger Dennis 'Denny' Peterson Doniver Ahlmĭuane Lawrence Elizabeth (Beth) Edstrom Ethel Schleh Faye Rousselow Gary Kneissel Jackie Tkadlec James Hendrix James Pojanowski Jerry Minder John Pitcher Joseph (Joe) Longtin Kathleen Webster Kenneth (Ken) Tolzmann Kevin Thomson Larry Guertin Larry Rainer

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